GSM4680579: anaerobically grown cells rep 3; Acidithiobacillus ferroo... - SRA

SRX8783924: GSM4680579: anaerobically grown cells rep 3; Acidithiobacillus ferrooxidans; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 4M spots, 766.6M bases, 500Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome profiling of hydrogen-oxidizing Acidithiobacillus ferrooxidans

PRJNA647821 • SRP273078 • All experiments • All runs

show Abstracthide Abstract

The study identified a total of 3169 gene transcripts (98.4% coverage). By comparing the anaerobic versus aerobic H2-oxidizing At. ferrooxidans cultures, a total of 371 DEGs were found. Of these, 168 DEGs were increased significantly during the aerobic growth on H2 (with O2 as the sole electron acceptor), while 203 DEGs increased significantly during anaerobic growth on H2 (with Fe3+ as the sole electron acceptor). Overall design: Transcriptome profiling of gene expression during aerobic and anaerobic growth of Acidithiobacillus ferrooxidans cells on hydrogen.

Sample: anaerobically grown cells rep 3

SAMN15590242 • SRS7052653 • All experiments • All runs

Organism: Acidithiobacillus ferrooxidans

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The total RNA was extracted with a TRI reagent (Sigma-Aldrich) and was treated with a TURBO DNA-free kit (Ambion) to remove the contaminating DNA. The cDNA libraries were constructed using the ScriptSeq™ Complete Kits (Bacteria) (Illumina), including Ribo-ZeroTM technology for ribosomal RNA removing and ScriptSeq v2 RNA-Seq Library Preparation Kit. Sequencing was performed on the Illumina MiSeq platform with MiSeq Reagent Kit v2 (500-cycles), which generated 250-bp paired-end reads.

Experiment attributes:

GEO Accession: GSM4680579

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 4M spots, 766.6M bases, 500Mb

Run	# of Spots	# of Bases	Size	Published
SRR12279150	3,986,163	766.6M	500Mb	2020-11-23

ID:: 11419191

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